Question

E. coli ribosomes contain three RNA molecules named for their sedimentation behavior: 5S, 16S, and 23S. Draw a diagram showing the approximate positions of the three RNA species following electrophoresis.

Short answer

Following electrophoresis, the following are the approximate positions of the three RNA species:

Step-by-step solution

Sedimentation

Sedimentation behavior characterizes the sedimentation level of DNA or RNA during centrifugation. It is used to display the molecule's relative molecular weight. The sedimentation coefficient of heavier RNA molecules is higher.

Diagram showing the approximate positions of the three RNA species

During gel electrophoresis, particles move through the gel matrix from negative to positive charge. Because heavier particles move slower than lighter ones, they travel a shorter journey compared to lighter ones.

Therefore, following electrophoresis, the approximate positions of the three RNA species will be: