Chapter 10: Q13CP (page 318)
Explain how an ABC transporter would operate as a flippase.
It is a transmembrane lipid and acts as flippase that belongs to the ABC transporter. For example, ATP-dependent pumps include a TP-binding cassette.
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A certain membrane protein allows phosphate groups to enter a eukaryotic cell.
(a) Would you expect the protein to function more like KcsA or more like GLUTI?
(b) Phosphate ions enter the cell along with ions. Describe this transport system using the terms introduced in Fig. 10-15.
(c) The eukaryotic intracellular pH is typically slightly lower than the extracellular pH. Does this suggest that the phosphate transporter carries out secondary active transport?
What are the similarities and differences among ionophores, porins, ion channels, and passive-mediated transport proteins? What determines the direction of solute movement?
Use the terminology of allosteric proteins to discuss the operation of proteins that carry out uniport, symport, and antiport transport processes.
For the problem in Sample Calculation 10-1, calculate ฮG at 37ยฐC when the membrane potential is (a) -50 mV (cytosol negative) and (b) +150 mV. In which case is Ca2+ movement in the indicated direction thermodynamically favorable?
How do transport proteins prevent the transmembrane movement of water and ions?
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