Question

You are attempting to determine KM by measuring the reaction velocity at different substrate concentrations, but you do not realize thatthe substrate tends to precipitate under the experimental conditions youhave chosen. How would this affect your measurement of KM?

Short answer

The experimentally determined KM will be very high than the calculated one.

Step-by-step solution

Enzymes

Enzymes are biological protein catalysts that change the rate of a process in a living organism. The molecule that binds to the enzyme's substrate binding site is referred to as the substrate.

Measurement of KMaffected when the substrate tends to precipitate

The substrate concentration will be lower if the substrate forms a precipitate under the experimental conditions. There won't be enough substrate to bind to the enzyme because there won't be enough of it. The reaction's velocity is affected as a result of this. As a result, the calculated KMwill be significantly higher than the experimentally determined one.

KM measurement will be affected when the substrate starts to precipitate.