Question

Is it necessary for measurements of reaction velocity to be expressed in units of concentration per time (M s⁻¹, for example) to calculate an enzyme's K_M?

Short answer

It is not necessary for the measurement of reaction velocity has been expressed in units of concentration per time to calculate the enzyme KM.

Step-by-step solution

Rate equation

The rate equation for a single-substrate enzyme-catalyzed reactionis known as the Michaelis–Menten equation. Through the Michaelis constant KM, a measure of substrate-binding affinity, this equation connects the starting reaction rate (v0), the maximal reaction rate (Vmax), and the initial substrate concentration [S].

The measurement of reaction velocity is not required

The rate of change per time is commonly used to calculate the velocity rate of change. Any appropriate Sl unit of velocity measurement can be utilised. KM stands for substrate concentration and is unaffected by velocity measurements. As a result, it is not required to describe reaction velocity measurementsin units of concentration per time when calculating an enzyme's KM.

Thus, the competitive inhibitorwas calculated on the basis of Michealis-Menten equation.