Question

Determine the type of inhibition of an enzymatic reaction from the following data collected in the presence and absence of the inhibitor.

[S] (mM)	vₒ (mM min⁻¹)	vₒ with I present (mM min⁻¹)
1	1.3	0.8
2	2.0	1.2
4	2.8	1.7
8	3.6	2.2
12	4.0	2.4

Short answer

The inhibitor is a mixed inhibitor.

Step-by-step solution

Biological protein catalysts

Enzymes are biological protein catalysts that change the rate of a process in a living organism. The molecule that binds to the enzyme's substrate binding site is referred to as the substrate. Enzyme inhibitors stop enzymes from functioning.

Inhibition of an enzymatic reaction

Both free enzyme and enzyme-substrate complexes bind to mixed inhibitors. The following data is used to create a Lineweaver-Burk graph. Extrapolation is carried out on the acquired lines.

A factor was added to theMichaelis-Menton equation. ∝ is a function of the concentration of a competitive inhibitor and the inhibitor's affinity for the enzyme. The concentration of an uncompetitive inhibitor and the inhibitor's affinity for the enzyme is used to calculate ∝ˈ. If there is an inhibitor, ∝ and ∝ˈ cannot be smaller than 1. Both with and without inhibitor are displayed on the graph. To the left of the 1/v axis, the lines cross.

Tabulation for plotting the graph

1/[S] (mM)	1/vₒ (mM min⁻¹)	1/vₒ with I present (mM min⁻¹)
1.0	0.7	1.2
0.5	0.5	0.8
0.3	0.4	0.6
0.1	0.3	0.5
0.08	0.2	0.4

Graphical representation of the Mixed inhibitor

Conclusion

Thus, the type of inhibition of an enzymatic reaction from the given data is collected in the presence and the absence of an inhibitor is mixed inhibition.