Chapter 25: Q5CP (page 897)
What is it important for DNA polymerase 1 to have three sites?
Short Answer
DNA polymerase enzyme synthesis a new complementary strand of DNA by
Incorporating SNMP sequentially in the S1 to 31 directions.
Chapter 25: Q5CP (page 897)
What is it important for DNA polymerase 1 to have three sites?
DNA polymerase enzyme synthesis a new complementary strand of DNA by
Incorporating SNMP sequentially in the S1 to 31 directions.
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A high rate of base-substitution mutations can result from a polymeraseโs tendency to incorporate a mispaired nucleotide or from its poor ability to incorporate the correct nucleotide. Use the following information about the catalytic efficiency (kcat/KM) of two error-prone polymerases to compare their ability substitution mutations.
Polymerase | Template nucleotide | Incoming nucleotide | kcat/KM (ยตM . min-1 ร 103 ) | |||
Polymerase ฦ | T | T | A | G | 420 | 22 |
HIV reverse transcriptase | T | T | A | G | 800 | 0.07 |
Describe the DNA Pol alpha, Pol S, PCNA, R Nose, H1, FENI, and DNA ligase participate in eukaryotic DNA synthesis?
What type of repair system would cells most likely use to repair DNA with a deaminated adenine?
Oxidative deamination of adenine produces hypoxanthine (the base of inosine), which can base pair with cytosine. (a) If no repair takes place, describe the makeup of the DNA in the two daughter cells following cell division. (b) Describe the makeup of the DNA in the four daughter cells following a second round of cell division.
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