Question

Summarize the function of the following protein in E-coli DNA

Replication: DNA polymerase I, DNA polymerase II, DNA helicase, SSB, primase, The sliding clamp, clamp loader, DNA ligase, Tus, and topoisomerase?

Short answer

The process of copying the DNA is termed as DNA Replication.

Step-by-step solution

Step 1: Requirement of DNA replication

There are many proteins required for DNA replication in eukaryotes such as DNA polymerase I, DNA polymerase II, DNA helicase, SSB, primase, The sliding clamp, clamp loader, DNA ligase, and topoisomerase.

Step 2: DNA  polymerase I

During DNA replication DNA polymerase I fill the DNA gap.

DNA polymerase II

It helps to synthesize DNA on both strands such as leading and lagging.

DNA helicase

It helps to separate the DNA which means the double-stranded DNAchangesinto single-stranded DNA.

SSB

Single-stranded DNA binding proteins attack single-stranded DNA and prevent DNA degradation.

Primase

During replication, the primary catalyzes the synthesis of small DNA and DNA in the same organisms.

The sliding clamp

It is a promoting factor during DNA replication.

Clamp loader

It is a loader pentameric ATPase that operates the process of DNA replication.

DNA ligase

It helps to join the nicked DNA.

Tus

It helps to bind terminatorsequencesand work as a counter helicase.

Topo isomerase

It helps to winding and unwinding of DNA.

Thus, all protein helps in DNA replication in E-coli.