Chapter 25: Q32CP (page 933)
What fraction of the human genome consists of transposons and retrotransposons?
The fraction of the human genome that consists of transposons and retrotransposons are 3/100 and 2/3 respectively.
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Why is it necessary to use only the Klenow fragment, rather than intact , in DNA sequencing reactions (Sections and )?
Two approaches for correcting single-gene defects are gene therapy such as is discussed in Sectionand thesystem. Explain why theapproach can potentially provide more complete restoration of normal tissue function than gene therapy.
Describe the enzymatic activities required for base excision repair, nucleotide excision ne mismatch repair.
The base analog 5-bromouracil (5BU),
which sterically resembles thymine, more readily undergoes tautomerization from its keto form to its enol form than does thymine. 5BU can be incorporated into newly synthesized DNA when it pairs with adenine on the template strand. However, the enol form of 5BU pairs with guanine rather than adenine. (a) Draw the 5BU · G base pair. (b) What type of mutation results?
In some experimental systems, a newly synthesized RNA primer is transferred from primase to the clamp or clamp loader rather than directly to DNA polymerase. Explain why this would maximize the efficiency of DNA replication
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