Chapter 25: Q31P (page 935)
What fraction of the human genome can sits of transposons and retro transposons?
Transposable elements (TEs) are DNA sequences that move from one location on the genome to another.
They are also called “jumping genes”
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What are the symptoms of defective nucleotaide excision the mismatch repair?
What type of repair system would cells most likely use to repair DNA with a deaminated adenine?
Explain why interstrand DNA cross-links are much more mutagenic than intrastrand cross-links.
A high rate of base-substitution mutations can result from a polymerase’s tendency to incorporate a mispaired nucleotide or from its poor ability to incorporate the correct nucleotide. Use the following information about the catalytic efficiency (kcat/KM) of two error-prone polymerases to compare their ability substitution mutations.
| Polymerase | Template nucleotide | Incoming nucleotide | kcat/KM (µM . min-1 × 103 ) | |||
| Polymerase ƞ | T | T | A | G | 420 | 22 |
| HIV reverse transcriptase | T | T | A | G | 800 | 0.07 |
Why can’t a linear duplex DNA, such as that of bacteriophage T7, be fully replicated by just E. coli-encoded proteins?
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