Chapter 25: Q21CP (page 915)
What are the functions of DNA phtolyases and alkytransfarses?
The enzymes Photolyase and alkytranferases are the phylogenetically old enzymes which is present and junctional in many species.
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Describe the enzymatic activities required for base excision repair, nucleotide excision ne mismatch repair.
In E. coli, all newly synthesized DNA appears to be fragmented (an observation that could be interpreted to mean that the leading strand as well as the lagging strand is synthesized discontinuously). However, inmutants that are defective in uracil–DNA glycosylase, only about half the newly synthesized DNA is fragmented. Explain.
Yeast and some other organisms produce proteins that closely resemble -alkylguanine–DNA alkyltransferase but lack a Cys at the active site. (a) Explain why these proteins cannot remove an alkyl group attached to guanine. (b) These proteins do protect cells against DNA alkylation damage. In which type of repair process are these proteins most likely to participate?
The E. coli genome contains 1009 Chi sequences. Do these sequences occur at random, and, if not, how much more or less frequently than random do they occur?
How many sliding clamps and clamp loading events are required for synthesis of the leading and lagging strands?
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