Those who inherit a mutant allele of the \(R B 1\) gene are at risk
for developing a bone cancer called osteosarcoma. You suspect that in these
cases, osteosarcoma requires a mutation in the second \(R B 1\) allele, and you
have cultured some osteosarcoma cells and obtained a cDNA clone of a normal
human \(R B 1\) gene.
A colleague sends you a research paper revealing that a strain of cancer-prone
mice develops malignant tumors when injected with osteosarcoma cells, and you
obtain these mice. Using these three resources, what experiments would you
perform to determine (a) whether osteosarcoma cells carry two \(R B 1\)
mutations,
(b) whether osteosarcoma cells produce any pRB protein, and
(c) if the addition of a normal \(R B 1\) gene will change the cancercausing
potential of osteosarcoma cells?