Chapter 11: Problem 5
What genetic process is occurring in a puff of a polytene chromosome?
Chapter 11: Problem 5
What genetic process is occurring in a puff of a polytene chromosome?
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Get started for freeWhat do SINE and LINE mean in terms of chromosome struc- ture? Why are they called "repetitive"?
Supercoiled DNA is slightly unwound compared to relaxed DNA and this enables it to assume a more compact structure with enhanced physical stability. Describe the enzymes that control the number of supercoils present in the \(E\) coli chromosome. How much would you have to reduce the linking number to increase the number of supercoils by five?
The human genome contains approximately \(10^{6}\) copies of an \(A l u\) sequence, one of the best-studied classes of short interspersed elements (SINEs), per haploid genome. Individual Alus share a 282-nucleotide consensus sequence followed by a 3'-adeninerich tail region (Schmid, 1998. Nucl. Acids Res. 26: 4541-4550). Given that there are approximately \(3 \times 10^{9}\) bp per human haploid genome, about how many base pairs are spaced between each Alu sequence?
A particular variant of the lambda bacteriophage has a DNA double-stranded genome of 51,365 base pairs. How long would this DNA be?
In an article entitled "Nucleosome Positioning at the Replication Fork," Lucchini and others (2002. EMBOJ. 20: 7294-7302) state, "both the 'old' randomly segregated nucleosomes as well as the 'new' assembled histone octamers rapidly position themselves (within seconds) on the newly replicated DNA strands." Given this statement, how would one compare the distribution of nucleosomes and DNA in newly replicated chromatin? How could one experimentally test the distribution of nucleosomes on newly replicated chromosomes?
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