Chapter 8: Problem 2
A number of these center around the findings that genetic recombination occurs in bacteria. Write a short summary that describes ways in which recombination may occur in bacteria.
Chapter 8: Problem 2
A number of these center around the findings that genetic recombination occurs in bacteria. Write a short summary that describes ways in which recombination may occur in bacteria.
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Get started for freeDescribe the temporal sequence of the bacteriophage life cycle.
In this chapter, we have focused on genetic systems present in bacteria and the viruses that use bacteria as hosts (bacteriophages). In particular, we discussed mechanisms by which bacteria and their phages undergo genetic recombination, the basis of chromosome mapping. Based on your knowl- edge of these topics, answer several fundamental questions: (a) How do we know that bacteria undergo genetic recombination, allowing the transfer of genes from one organism to another? (b) How do we know that conjugation leading to genetic recombination between bacteria involves cell contact, which precedes the transfer of genes from one bacterium to another? (c) How do we know that during transduction bacterial cell-tocell contact is not essential?
In the plaque assay, exactly what makes up a single plaque?
Distinguish among the three modes of recombination in bacteria.
A plaque assay is performed beginning with \(1.0 \mathrm{mL}\) of a solution containing bacteriophages. This solution is serially diluted three times by taking \(0.1 \mathrm{mL}\) and adding it to \(9.9 \mathrm{mL}\) of liquid medium. \(0.1 \mathrm{mL}\) of the final dilution is plated and yields 17 plaques. What is the initial density of bacteriophages in the original \(1.0 \mathrm{mL} ?\)
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