Chapter 10: Problem 19
If the analysis of DNA from two different microorganisms demonstrated very similar base compositions, are the DNA sequences of the two organisms also nearly identical?
Chapter 10: Problem 19
If the analysis of DNA from two different microorganisms demonstrated very similar base compositions, are the DNA sequences of the two organisms also nearly identical?
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Get started for freeDefine and indicate the significance of (a) Okazaki fragments, (b) DNA ligase, and (c) primer RNA during DNA replication.
Several temperature-sensitive mutant strains of \(E .\) coli display the following characteristics. Predict what enzyme or function is being affected by each mutation. (a) Newly synthesized DNA contains many mismatched base pairs. (b) Okazaki fragments accumulate, and DNA synthesis is never completed. (c) No initiation occurs. (d) Synthesis is very slow. (e) Supercoiled strands remain after replication, which is never completed.
List and describe the function of the ten subunits constituting DNA polymerase III. Distinguish between the holoenzyme and the core enzyme.
Kornberg showed that nucleotides are added to the 3 '-end of each growing DNA strand. In what way does an exposed \(3^{\prime}\) - OH group participate in strand elongation?
In this chapter, we focused on how DNA is replicated and synthesized. In particular, we elucidated the general mechanism of replication and described how DNA is synthesized when it is copied. Based on your study of these topics, answer the following fundamental questions: (a) What is the experimental basis for concluding that DNA replicates semiconservatively in both bacteria and eukaryotes? (b) How was it demonstrated that DNA synthesis occurs under the direction of DNA polymerase III and not polymerase I? (c) How do we know that in vivo DNA synthesis occurs in the \(5^{\prime}\) to \(3^{\prime}\) direction? (d) How do we know that DNA synthesis is discontinuous on one of the two template strands? (e) What observations reveal that a "telomere problem" exists during eukaryotic DNA replication, and how did we learn of the solution to this problem?
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