Chapter 5: Problem 4
Why does more crossing over occur between two distantly linked genes than between two genes that are very close together on the same chromosome?
Chapter 5: Problem 4
Why does more crossing over occur between two distantly linked genes than between two genes that are very close together on the same chromosome?
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Get started for freeIn Drosophila, a cross was made between females-all expressing the three \(X\) -linked recessive traits scute bristles \((s c),\) sable body \((s),\) and vermilion eyes \((v)-\) and wild-type males. In the \(\mathrm{F}_{1},\) all females were wild type, while all males expressed all three mutant traits. The cross was carried to the \(\mathrm{F}_{2}\) generation, and 1000 offspring were counted, with the results shown in the following table. No determination of sex was made in the data. (a) Using proper nomenclature, determine the genotypes of the \(P_{1}\) and \(F_{1}\) parents. (b) Determine the sequence of the three genes and the map distances between them. (c) Are there more or fewer double crossovers than expected? (d) Calculate the coefficient of coincidence. Does it represent positive or negative interference?
List some of the differences between a linkage map obtained by analyzing crossovers and a physical map obtained by sequencing the DNA.
The gene controlling the Xg blood group alleles \(\left(X g^{+} \text {and } X g^{-}\right)\) and the gene controlling a newly described form of inherited recessive muscle weakness called episodic muscle weakness \((E M W X)\) (Ryan et al., 1999 ) are closely linked on the X chromosome in humans at position \(\mathrm{Xp} 22.3\) (the tip of the short arm \() .\) A male with EMWX who is \(\mathrm{Xg}^{-}\) marries a woman who is \(\mathrm{Xg}^{+}\), and they have eight daughters and one son, all of whom are normal for muscle function, the male being \(\mathrm{Xg}^{+}\) and all the daughters being heterozygous at both the \(E M W X\) and \(X g\) loci. Following is a table that lists three of the daughters with the phenotypes of their husbands and children. (a) Create a pedigree that represents all data stated above and in the following table. (b) For each of the offspring, indicate whether or not a crossover was required to produce the phenotypes that are given.
In this chapter, we focused on linkage, chromosomal mapping, and many associated phenomena. In the process, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions? (a) How was it established experimentally that the frequency of recombination (crossing over) between two genes is related to the distance between them along the chromosome? (b) How do we know that specific genes are linked on a single chromosome, in contrast to being located on separate chromosomes? (c) How do we know that crossing over results from a physi- cal exchange between chromatids? (d) How do we know that sister chromatids undergo recombination during mitosis? (e) When designed matings cannot be conducted in an organism (for example, in humans), how do we learn that genes are linked, and how do we map them?
Are mitotic recombinations and sister chromatid exchanges effective in producing genetic variability in an individual? in the offspring of individuals?
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