Chapter 19: Problem 6
Can cancer be inherited or infectious?
Chapter 19: Problem 6
Can cancer be inherited or infectious?
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Mutations in tumor-suppressor genes are associated with many types of cancers. In addition, epigenetic changes (such as DNA methylation) of tumor-suppressor genes are also associated with tumorigenesis (Otani et al., 2013. Expert Rev Mol Diagn \(13: 445-455\) ). (a) How might hypermethylation of the \(p 53\) gene promoter influence tumorigenesis? (b) Knowing that tumors release free DNA into certain surrounding body fluids through necrosis and apoptosis (Kloten et al., 2013. Breast Cancer Res. \(15(1): \mathrm{R} 4\) ), outline an experimental protocol for using human blood as a biomarker for cancer and as a method for monitoring the progression of cancer in an individual.
How do translocations such as the Philadelphia chromosome contribute to cancer?
A study by Bose and colleagues (1998. Blood 92: \(3362-3367\) ) and a previous study by Biernaux and others (1996. Bone Marrow Transplant 17: (Suppl.3) \(\mathrm{S} 45-\mathrm{S} 47\) ) showed that \(B C R-A B L\) fusion gene transcripts can be detected in 25 to 30 percent of healthy adults who do not develop chronic myelogenous leukemia (CML). Explain how these individuals can carry a fusion gene that is transcriptionally active and yet do not develop CML.
Those who inherit a mutant allele of the \(R B 1\) gene are at risk for developing a bone cancer called osteosarcoma. You suspect that in these cases, osteosarcoma requires a mutation in the second \(R B 1\) allele, and you have cultured some osteosarcoma cells and obtained a cDNA clone of a normal human \(R B I\) gene. A colleague sends you a research paper revealing that a strain of cancer-prone mice develop malignant tumors when injected with osteosarcoma cells, and you obtain these mice. Using these three resources, what experiments would you perform to determine (a) whether osteosarcoma cells carry two \(R B 1\) mutations, (b) whether osteosarcoma cells produce any \(\mathrm{pRB}\) protein, and (c) if the addition of a normal \(R B 1\) gene will change the cancer-causing notential of astensarcama cells?
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