Chapter 18: Problem 8
List the main classes of zygotic genes. What is the function of each class of these genes?
Chapter 18: Problem 8
List the main classes of zygotic genes. What is the function of each class of these genes?
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Get started for freeDistinguish between the syncytial blastoderm stage and the cellular blastoderm stage in Drosophila embryogenesis.
The floral homeotic genes of Arabidopsis belong to the MADSbox gene family, while in Drosophila, homeotic genes belong to the homeobox gene family. In both Arabidopsis and Drosophila, members of the Polycomb gene family control expression of these divergent homeotic genes. How do Polycomb genes control expression of two very different sets of homeotic genes?
Embryogenesis and oncogenesis (generation of cancer) share a number of features including cell proliferation, apoptosis, cell migration and invasion, formation of new blood vessels, and differential gene activity, Embryonic cells are relatively undifferentiated, and cancer cells appear to be undifferentiated or dedifferentiated. Homeotic gene expression directs early development, and mutant expression leads to loss of the differentiated state or an alternative cell identity. M. T. Lewis (2000. Breast Can. Res. \(2: 158-169\) ) suggested that breast cancer may be caused by the altered expression of homeotic genes. When he examined 11 such genes in cancers, 8 were underexpressed while 3 were overexpressed compared with controls. Given what you know about homeotic genes, could they be involved in oncogenesis?
Much of what we know about gene interactions in development has been learned using nematodes, yeast, flies, and bacteria. This is due, in part, to the relative ease of genetic manipulation of these well-characterized genomes. However, of great interest are gene interactions involving complex diseases in humans. Wang and White (2011. Nature Methods 8(4) \(341-346\) ) describe work using RNAi to examine the interactive proteome in mammalian cells. They mention that knockdown inefficiencies and off-target effects of introduced RNAi species are areas that need particular improvement if the methodology is to be fruitful. (a) How might one use RNAi to study developmental pathways? (b) Comment on how "knockdown inefficiencies" and "off-tar-get effects" would influence the interpretation of results.
In Arabidopsis, flower development is controlled by sets of homeotic genes. How many classes of these genes are there, and what structures are formed by their individual and combined expression?
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