Problem 1
_________cut(s) DNA molecules at specific sites. a. DNA polymerase b. DNA probes c. Restriction enzymes d. DNA ligase
Problem 2
A_________ is a molecule that can be used to carry a fragment of DNA into a host organism. a. cloning vector b. chromosome c. GMO d. PCR
Problem 3
For each species, all ________in the complete set of chromosomes is the___________. a. mutations; DNA profile b. DNA; genome c. SNPs; genome d. genomes; genotype
Problem 4
A set of cells that host various DNA fragments collectively representing an organism's entire set of genetic information is called a __________ a. genome b. DNA library c. clone d. polymorphism
Problem 5
PCR is often used ________ a. in DNA profiling b. to modify a human genome c. as a cloning vector
Problem 6
Fragments of DNA can be separated by electrophoresis according to __________. a. sequence b. length c. species d. SNPs
Problem 7
\(Taq\) polymerase is used for PCR because it ____________. a. tolerates the high temperature needed to separate DNA strands b. is an enzyme from a bacterium c. does not require primers d. is genetically modified
Problem 8
__________is a technique to determine the order of nucleotides in a fragment of DNA. a. PCR b. DNA sequencing c. Electrophoresis d. Nucleic acid hybridization
Problem 9
An individual's unique set of _________ can be used in DNA profiling. a. DNA sequences b. short tandem repeats c. SNPs d. all of the above
Problem 10
Put the following tasks in the order they would occur during a cloning experiment. a. Using DNA ligase to seal DNA fragments into vectors b. Using a probe to identify a clone in the library c. Sequencing the DNA of the clone d. Making a DNA library of clones e. Using restriction enzymes to cut chromosomal DNA into fragments
