Question

Which of the following types of molecules could bind to an ncRNA through base pairing? a. DNA b. RNA c. protein d. small molecule e. both a and b

Short answer

The types of molecules that could bind to an ncRNA through base pairing are both a (DNA) and b (RNA).

Step-by-step solution

Analyze the binding through base pairing

The exercise refers to molecules binding to ncRNA (non-coding RNA) through base pairing. Base pairing is a specific form of interaction that occurs between nucleic acids (DNA and RNA) where certain nucleotides in one strand bond to certain nucleotides in the second strand. The pairs are adenine (A) with thymine (T) in DNA, and adenine with uracil (U) in RNA, and cytosine (C) with guanine (G).

Analyzing the options

The exercise provides five options for potential binders: DNA, RNA, proteins, small molecules, and both DNA and RNA. Considering the concept of base pairing, we understand that this form of interaction occurs between nucleic acids. Proteins, although capable of interacting with ncRNA, they do not base pair with it. The same applies to small molecules.

Choose the correct answers

With the information obtained in the previous steps, it can be concluded that the molecules that can base pair with ncRNA are DNA and RNA. Therefore, the correct answers are both a and b.

Key concepts

Understanding Non-Coding RNA

Non-coding RNA (ncRNA) represents a class of RNA molecules that do not encode for proteins but are crucial for regulating various biological processes. ncRNAs come in various forms, from transfer RNA (tRNA) and ribosomal RNA (rRNA), to microRNA (miRNA) and long non-coding RNA (lncRNA).

These molecules play roles in gene expression regulation, RNA splicing, and epigenetic modifications, among others. Unlike messenger RNA (mRNA), which is used as a template for protein synthesis, ncRNAs perform their functions without being translated into proteins. Instead, they often exert their influence through interactions with other nucleic acids, proteins, or even by modifying the structure of the genome itself.

Nucleic Acid Interactions

Nucleic acid interactions are the cornerstone of genetic function and regulation. These interactions can be between two strands of DNA, RNA with RNA, as well as between DNA and RNA. These interactions are usually specific and follow the rules of base pairing, where complementary nucleotide bases form hydrogen bonds with one another. As it is put succinctly in the textbook example, base pairing allows nucleic acids to form complex structures and engage in processes like DNA replication, transcription, and RNA interference.

The specificity of these interactions enables the accurate transmission of genetic information and the complex regulation of gene expression. These interactions also allow for the formation of unique three-dimensional structures, which are critical for the function of ncRNAs in their various roles within the cell.

Base Pairing Specificity

Base pairing specificity refers to the precise way in which nucleotide bases interact with one another. In the cells, adenine (A) pairs with thymine (T) in DNA and with uracil (U) in RNA, while cytosine (C) pairs with guanine (G). These are known as complementary base pairs and are held together by hydrogen bonds - A with T or U forms two hydrogen bonds, while C and G form three. This specific pairing is crucial for the structure and integrity of DNA and RNA molecules.

When considering non-coding RNA (ncRNA), this specificity ensures that ncRNA can form complex secondary and tertiary structures. It also allows ncRNA to selectively bind to other nucleic acids, which is essential for carrying out their regulatory functions. In the context of the original exercise, the recognition that DNA and RNA can bind to ncRNA via base pairing underscores the importance of this specificity to molecular biology and genetics.