Chapter 15: Q.27 (page 393)
Transcribe and translate the following DNA sequence (nontemplate strand): 5'-ATGGCCGGTTATTAAGCA-3'
Short Answer
The mRNA would be: 5' - AUGGCCGGUUAUUAAGCA - 3'
Chapter 15: Q.27 (page 393)
Transcribe and translate the following DNA sequence (nontemplate strand): 5'-ATGGCCGGTTATTAAGCA-3'
The mRNA would be: 5' - AUGGCCGGUUAUUAAGCA - 3'
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Get started for freeA fragment of bacterial DNA reads: 3โ โTACCTATAATCTCAATTGATAGAAGCACTCTACโ 5โ Assuming that this fragment is the template strand, what is the sequence of mRNA that would be transcribed? (Hint: Be sure to identify the initiation site.)
A scientist introduces a mutation that makes the 60S ribosomal subunit nonfunctional in a human cell line. What would be the predicted effect on translation?
a. Translation stalls after the initiation AUG codon is identified.
b. The ribosome cannot catalyze the formation of peptide bonds between the tRNAs in the A and P sites.
c. The ribosome cannot interact with mRNAs.
d. tRNAs cannot exit the E site of the ribosome.
Which feature of promoters can be found in both prokaryotes and eukaryotes?
a. GC box
b. TATA box
c. octamer box
d. -10 and -35 sequences
If mRNA is complementary to the DNA template strand and the DNA template strand is complementary to the DNA nontemplate strand, then why are base sequences of mRNA and the DNA nontemplate strand not identical? Could they ever be?
A scientist observes that a cell has an RNA polymerase deficiency that prevents it from making proteins. Describe three additional observations that would together support the conclusion that a defect in RNA polymerase I activity, and not problems with the other polymerases, causes the defect.
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