Question

In which direction does DNA replication take place? a. 5'-3' b. 3'-5' c. 5' d. 3'

Short answer

The synthesis of DNA is always in the$5^{\text{'}}-3^{\text{'}}.$ because the DNA polymerase enzyme can only add nucleotides at the end (a).

Step-by-step solution

Step:1 Introduction

Semi-conservative replication of DNA results in the formation of two daughter DNA molecules, each with a parental strand and a newly synthesized strand.

Step:2 Correct answer

Replication of DNA is always done in the 5'-3' orientation. As a result, option (a) is correct.

Sttep:3 Justification for the correct answer

Option (a) is denoted by the letter DNA polymerase is an enzyme that adds nucleotides to existing strands during $5^1-3^1$ DNA replication. The activity of DNA polymerase is present. As a result, DNA polymerase adds nucleotides to the RNA primer's free hydroxyl group when the DNA template is read in the to direction. As a result, the new strand's synthesis progresses in the same direction.

Step:4 Explanation of incorrect answer

3'to 5'is the range for option (b). The DNA template is read in this direction, although DNA synthesis occurs in the 5'-3' direction. As a result, it is an inaccurate response.

Option (c) is denoted by the number 5'. 5'-3' is where DNA is synthesized. As a result, it is an inaccurate response.

Option (d) is denoted by the number 3'. 5'-3' is where DNA is synthesized. As a result, it is an inaccurate response.

As a result, alternatives (b), (c), and (d) are wrong.